Mat Titers Leptospirosis

Sera are screened at a 1 100 dilution and those showing agglutination are then serially diluted further to determine a titer endpoint.

Mat titers leptospirosis.

Does the mat always work. As with other serology assays vaccination complicates the interpretation of titers. An interpretation is included in each mat report at no extra charge and additional consultation is also available. Laboratory studies can confirm the diagnosis and determine the extent of organ involvement.

If the level of antibodies called a titer is high enough or can be shown to be rising over time then infection is confirmed. Testing may include culture and or serology. The mat or microscopic agglutination test detects the presence of antibodies against leptospira in a dog s blood. Horses that abort due to leptospirosis exposure tend to have very high mat titers.

The mat is the keystone for a correct diagnosis wolff 1954. For the reading of mat titers it is highly recommended to use a darkfield microscope with a high contrast between the leptospires and the dark background. Detection of antibodies using the microscopic agglutination test mat has been the most common diagnostic method used for the diagnosis of canine leptospirosis 10 vaccination with commercially available leptospirosis vaccines will produce detectable mat titers 10 see algorithm 2 for more information on mat results. Human leptospirosis is an acute febrile illness that presents with many manifestations and is found worldwide.

The mat is an excellent test but it. Titers of greater than 100 are usually considered significant although these may be due to vaccination. Results are expressed as the reciprocal of the highest dilution where agglutination is observed. What is the mat test for leptospirosis.

For all species of animals when chronic leptospirosis is suspected abortion anterior uveitis hepatitis and nephritis etc it is not necessary to collect a convalescent serum sample. In equine abortion cases a mat titer of 1 6400 or higher is considered. For all 108 patients leptospirosis was diagnosed on the basis of a fourfold or greater increase in titer by mat seroconversion and all but 1 of 245 controls were mat negative titers 1 100. A single elevated mat titer in a patient with a compatible febrile illness and suspected exposure suggests acute leptospirosis.

Major disadvantages of microscopic agglutination test mat. Titers against serovar autumnalis are available in our expanded panel test code 95066 or as an add on test code 95054 by request. A severe form which includes jaundice kidney failure and bleeding is called weil disease. A series of cultures of different leptospires are incubated with serial dilutions of serum.